Type IIS restriction endonucleases mediated cloning
Type IIS-mediated assembly methods are based on the original description of "Golden Gate" cloning (Engler et al. 2008) and allows efficient simultaneous assembly of multiple DNA parts, without requiring the isolation of individual fragments.
How type IIS restriction enzymes enable a one-pot reaction
How to easily and reliably assemble multiple standard parts in a single reaction, and
How to make new parts
Type IIS methods in plants