Type IIS restriction endonucleases mediated cloning

Type IIS-mediated assembly methods are based on the original description of "Golden Gate" cloning (Engler et al. 2008) and allows efficient simultaneous assembly of multiple DNA parts, without requiring the isolation of individual fragments.

Nicola Patron has assembled a short online course on Type IIS that covers:

  • How type IIS restriction enzymes enable a one-pot reaction

  • How to easily and reliably assemble multiple standard parts in a single reaction, and

  • How to make new parts

An introduction to Type IIS restriction enzymes and how they differ from classical typeII restriction enzymes.

Type IIS methods in plants

  • MoClo

  • GoldenBraid

  • Loop Assembly