Loop type IIS protocols

1- Loop Assembly

Developed in Jim Haseloff Lab by Bernardo Pollack and Fernan Federici, the manuscript is already available in bioRxiv.

Odd receivers:

pOd1, pOd2, pOd3, pOd4

Cloning into with BsaI

Low copy number pGreen-based plasmids

Confer KanR

Cloning into with BsaI

To assemble L1 contructs (and L3, L5...)

Cloning out with SapI

EVEN receivers:

pEv1, pEv2, pEv3, pEv4

Low copy number pGreen-based plasmids

Confer SepcR

Cloning into with SapI

To assemble L2 contructs (and L4, L6..)

Paper in bioRxiv here

Paper in bioRxiv here

Loop 2.0

Continuation of Loop by Susana Sauret-Gueto, Eftychios Frangedakis, Marta Tomaselli, Anthonty West, Nicola Patron, Jim Haseloff.

L1 receivers:

pCk1, pCk2, pCk3, pCk4

Higher copy numnber pCambia-based plasmids

Confer KanR

Cloning into with BsaI

To assemble L1 contructs (and L3, L5...)

Cloning out with SapI

L2 receivers:

pCsA, pCsB, pCsC, pCsD

Higher copy number pCambia-based plasmids

Confer SepcR

Cloning into with SapI

To assemble L2 contructs (and L4, L6..)

Cloning into with BsaI