OpenPlant Marchantia library of L0 parts
We are building the Marchantia library of L0 parts following the common syntax rules, using standard fusion sites depending on the type of part.
We are using Loop to assemble L1 and L2 constructs. Loop is an efficient and simple type IIS assembly method that uses BsaI and SapI as type IIS enzymes. Thus, the Marchantia L0 parts are domesticated for BsaI and SapI. More details on Loop here.
The L0 parts are being cloned into the pUAP1 plasmid, which is under openMTA. The pUAP1 plasmid has BsaI sites to digest out the part and assemble it with the other L0 parts in order to make a L1 construct. pUAP1 in Addgene here.
We are building a new version of pUAP1 in order to clone into it using SapI. More news on this soon...
The library of Marchantia parts includes:
Registry of Mp L0 parts
We are using Benchling as our electronic lab notebook, to edit and annotate plasmids, do assemblies and register the collection of constructs in standard ways using schemas with predefined fields.
We are looking into ways of moving parts from the Haseloff Lab registry into the public domain once they are tested, as well as on platforms that would allow integrating the experience/results of different users when using the collection of parts. If you have feedback around these topics, you are welcomed to contact us.
Contribute parts to the kit
At the moment most of the parts come from the Haseloff Lab, but we are collaborating with other researchers/ groups, so if you'd like to contribute, you are welcomed to contact us.